RNA polymerase II (RNAPII) transcription is an immensely complex process, involving steps from initiation and elongation to termination, each requiring a diverse cohort of proteins that assemble on chromatin with precise spatiotemporal coordination. Single-molecule approach allows the characterization of molecular trajectories one at a time, from their diffusive search to chromatin engagement and protein interaction in living cells. My postdoctoral work involved developing single-molecule spatiotemporal mapping techniques in living yeast to uncover the diffusive confinement of RNA polymerase II around active genes. This confinement, which depends on its disordered C-terminal domain (CTD), facilitates an effective search on chromatin targets for transcription activation (link). My research currently focuses on addressing the dynamic landscape of RNAPI, RNAPII, and RNAPIII transcription-associated protein interactions in the nuclear space with sub-second resolution, and on pushing the state of the art in single-molecule imaging.